Abstract
Background: Leucocyte- and platelet-derived bioactive substances accumulated in blood preparations during procession and storage may play a role in side-effects, including immunosuppression, observed after transfusion. Leucodepletion by filtration may significantly reduce the amount of storage time-dependent accumulated substances. Therefore we studied the effect of pre-storage leucofiltration of red blood cell suspensions on stimulation of tumour necrosis factor-alpha (TNF-α) and interleukin-2 (IL-2) release in vitro. Materials and Methods: Nine units of CPDA-1 blood (whole blood suspended in citrate phosphate dextrose adenine-1), 9 units of pre-storage leucofiltered CPDA-1 blood, 9 units of SAGM blood (buffy coat-depleted red blood cells suspended in saline adenine glucose mannitol) and 9 units of pre-storage leucofiltered SAGM blood were stored for 35 days. Supernatants were collected from all units after storage for 1, 21 and 35 days. Assays of whole blood obtained from healthy volunteers with addition of supernatants from stored blood components were stimulated with lipopolysaccharide (LPS) or phytohemagglutinin A (PHA) and incubated for 24 h or 72 h. Control assays without addition of supernatants from stored blood components were run simultaneously. After incubation TNF-α and IL-2 concentrations were determined by ELISA methods in assay supernatants. Results: The stimulated TNF-α and IL-2 releases were significantly decreased by supernatants from CPDA-1 blood and SAGM blood in a storage time-dependent manner compared with release in control assays. Pre-storage leucofiltration of CPDA-1 blood improved TNF-α release significantly while leucofiltration of SAGM blood increased the release to concentrations higher than that observed in control assays. However, pre-storage leucofiltration had no effect on storage time-dependent decreased IL-2 release. Con-clusion: Pre-storage leucofiltration may improve certain in vitro immune responses impaired by supernatants from stored blood while others appear to be unaffected.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
