Abstract
The extraction of compounds from Stevia rebaudiana leaves under pressurized conditions was investigated. The effect of the ethanol percentage in the extracting solvent, static time, pressure and temperature on global extraction yield (YGE), total phenolic content (TPC) and antioxidant activity (AA) was evaluated. The extract from pressurized liquid extraction (PLE) was characterized and compared to that obtained from Soxhlet and ultrasound-assisted extraction (UAE). It appears that the increase of ethanol concentration in the solvent favored response variables, while the increase in static time and pressure did not affect the extraction. The temperature (125 ºC) provided an increase in YGE and the highest levels of active compounds were obtained after using 70% (v/v) ethanol in the extracting solvent (15 mL g−1) and 30 min. The different techniques evaluated provided similar YGE, while PLE providing greater extraction of TPC and AA, and Soxhlet favored the extraction of steviol glycosides and total flavonoids.
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