Abstract
SummaryC-L crystals concentrated and preserved as described did not differ from freshly collected crystals in histochemical reactions or solubility when kept over a period of 9 months. Thus it was feasible to accumulate a quantity of crystals sufficient for biochemical determinations.Advantage was taken of the solubility of the crystals at pH 2.4 to separate them from residual extraneous material. Histochemical reactions, absorption spectrum, biochemical tests and molecular weight studies suggest that the material composing C-L crystals is a polypeptide of low molecular weight containing tyrosine or tryptophane or both. It is soluble at pH 2 and pH 11.2. It was possible to recrystallize a dilute (0.5%) solution of C-L crystals by adjusting the solution to neutrality. No measurable amounts of phosphorus or zinc could be detected by spectrographic analysis.
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