Abstract
Any microbial process requires a reliable source of culture stock that is reproducible, viable and taxonomically determined and also preserved without changes in genetic and phenotypic traits. Here we have described most effective and reproducible method for preservation of bacterial spores on filter disc at -20°C, 4°C and RT for use in laboratory for microbial process. The filter disc is used as base to support the spores inside the tiny holes. The filter paper discs are dipped into the medium after the spore phase growth of microorganisms. The whatman filter wet discs are dried at 55-60°C till they are completely dry. The viability of discs stored at room temperature and -20°C were checked at 3, 4, 6 and 2 month intervals. This is cost effective technique. In absence of preservative, the viability of the cultures were checked on liquid medium and monitored for the production of the biomolecules such as cellulase. These techniques could be used for other bacterial spore species.
Highlights
The role of culture collection is to preserve and maintain microbial cultures without alteration of genetic or phenotypic characteristic of strain as source of microbial cell for various biotechnological processes.There are various preservation methods described and is used for microbial cultures for short and long term preservation
Bacterial growth medium such as glucose, yeast extract, peptone, were purchased from SRL laboratory, whatman filter paper was purchased from Millipore
Growth medium was inoculated with 2-3 pieces of filter discs
Summary
There are various preservation methods described and is used for microbial cultures for short and long term preservation. Method for preservation of fungal culture using modified filter paper technique for long term preservation was described by Fong et al [1]. Some preservation methods have limitations such as time consuming, cost effective and genetic and physiological variations during long term maintenance [2]. There are various methods of preservation of the bacterial cultures described.
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