Abstract
Three methods for preserving 16 Streptornyces strains were tested: lyophilization on soil and storage in frozen glycerol medium (-20°C), and in liquid nitrogen (-196°C) in the presence of dimethyl sulfoxide. The viability, stability of auxotrophic markers, antibiotic production, and resistance to their own antibiotics for a period up to one year were studied. No variations in the production of and resistance to antibiotics or accumulation of revertants of mutants were evident during the toral preservation time in all three storage methods. A drastic decrease in viable counts was observed after lyophilization on soil. Viability of strains frozen in glycerol and after storage in liquid nitrogen was similar and ranged from 2.3% to 36.6%.Storage of streptomycetes in frozen glycerol is recommended as a quick and reliable method for frequent studies in the laboratory. Storage in liquid nitrogen is recommended as a long-term preservation method.
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