Abstract
BackgroundThe aim of the present study was to characterize the morphological and ultrastractural of mouse ovarian tissue with different cryoprotectant solution.ObjectiveAim of this study, is to demonstrae an improved convetional vitrification method on mouse ovarian tissue using different concentrations of ethylene glycol (EG) and/or dimetyl sulfoxide (DMSO) and EG.Materials and methodsMouse ovarian tissue dissected and were randomly assigned to three groups: control, conventional vitrification (CV) and toxicity test. Then ovaries were vitrified by 5%, 10% EG or DMSO CV1-CV4, 5%, 10% EG plus DMSO CV5-CV6 and EG plus DMSO in climbing concentrations CV7. The effect of cryoprotectant solutions on ovarian tissue were evaluated by histological examination hematotoxillin & eosin stain, H&E, viability assessment trypan blue stain and ultrastructural analyses transmission electron microscopy, TEM.ResultsOvarian tissue frozen in CV7 solution showed a higher percentage of morphologically normal follicles or viable follicles than other cryoprotectant solutions P < 0.05. Ultrastructural analysis of ovarian tissue showed that less damage was observed in CV7 and it was very similar to the control group.ConclusionVitrification of ovarian tissue with optimal cryoprotectant solutions such as EG plus DMSO is the most effective for preserving the structural efficiency of ovarian follicles.
Highlights
The aim of the present study was to characterize the morphological and ultrastractural of mouse ovarian tissue with different cryoprotectant solution
Ovarian tissue frozen in CV7 solution showed a higher percentage of morphologically normal follicles or viable follicles than other cryoprotectant solutions P < 0.05
Ultrastructural analysis of ovarian tissue showed that less damage was observed in CV7 and it was very similar to the control group
Summary
The aim of the present study was to characterize the morphological and ultrastractural of mouse ovarian tissue with different cryoprotectant solution. The cryopreservation of ovarian tissue is a suitable technique to reservation the fertility of women and young girls before undertaking chemotherapy or radiotherapy and high risk for premature and genetic mutations [1]. In. Recently EG and DMSO have been used by numerous authors as cryoprotectants for cryopreservation of ovarian tissue [8,9]. Viable tissue has been obtained after conventional freezing with DMSO and EG [1]. The vitrification technique and cryoprotectants agents are major factors in the cryopreservation of ovarian tissue. Because of the main challenge is to find the optimum composition for a cryoprotectant solution having as little a biologic toxicity and damage for ovarian tissue
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