Abstract
A method for freezing preservation of the filamentous cyanobacteria Pseudanabaena galeata Böcher and Geitlerinema amphibium (Ag. ex Gom.) Anagn. at −30°C and −80°C was examined. Four freezing procedures, two different cryoprotectors and two growth states were assayed. The use of 5% v/v dimethyl sulphoxide as cryoprotectant produced better results than methanol 1.5 M. In general, recoveries over all the storage temperatures and freezing procedures tested were higher in G. amphibium than in P. galeata, which was more sensitive to intercellular trichome breakage. The storage of P. galeata and G. amphibium cultures in stationary and logarithmic growth phase respectively, with 5% v/v dimethyl sulphoxide at −30°C or −80°C, proved to be a suitable conservation technique for these cyanobacteria.
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