Preservation Effect of Crude Fig Fruit Filtrate (Ficus carica L) Added In to Tris Egg Yolk Based Extender on Capacitating, Acrosome and Fertility of Half Blood Boer Buck Spermatozoa

Abstract

This research was conducted to know the effect of additional crude filtratet of fig fruit (CF3) into tris egg yolk (TEY) based extender on sperm progressively motile, plasma membrane integrity, incapacitation, intact acrosome and fertility of half blooded Boer buck liquid semen. Ten ejaculates were collected by artificial vagina every four days. The collected semen was divided into four aliquots in accordance to the treatments extender (0%, 4%, 5% and % CF3 in TEY based extender, v/v, respectively). The overall percentage of progressively motile sperm were significantly higher (P<0.05) in TEY + 6% CF3 as compared to control and 4% CF3 but was not significantly different as compared to 5% CF3. The overall percentages of sperm with intact plasma membrane was significantly higher (P<0.05) in TEY based extender + 6% CF3 as compared to the control, 4% and 5% CF3. The percentage difference between the incapacitated spermatozoa were significantly higher (P<0.05) for both in 5% and 6% CF3 since 0 to 96 hours stored at 5°C as compared to control and 4%. The difference between the overall mean percentages of spermatozoa with intact acrosome in TEY based extender incorporated with 5% and 6% CF3 were significantly higher (P<0.05) than control and 4% CF3. Pregnancy rates to AI using only preserved semen at 5°C for 96 hours, extended in TEY based extender + 6% CF3 was 44.68%. It is concluded that additional CF3 into TEY based extender increased preservation ability of extender in liquid form of buck semen up to 96 hours at 5°C in TEY + 6% CF3.