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Abstract
The effectiveness of glycerol and ethylene glycol in preserving the viability of tooth germs subjected to various freezing temperatures was investigated by explanting the tissues, after thawing, to tissue cultures, and observing the extent of cellular outgrowth and the degree of internal differentiation. Tooth germs were pretreated with various concentrations of the preservative agents, frozen indirectly in dry ice-alcohol solutions (−78 °C), isopentane cooled in liquid nitrogen (about −120 °C), or liquid nitrogen (−198 °C), and cultured in Carrel flasks. Glycerol in 30% solution appeared to be the most effective pretreating agent, and the slowest rate of freezing (dry ice-alcohol at −78 °C) was the least damaging of the freezing rates employed.
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