Abstract
The objective of this study was to test the efficiency of preservation and maceration methods for Euterpe precatoria leaflet tissue to obtain genomic DNA for molecular studies. The leaflets of E. precatoria were collected in an experimental field at Embrapa Acre, Brazil. The study was conducted in a completely randomized design with 10 replicates, in a 12 × 2 factorial structure, with 12 storage treatments (fresh; lyophiliser 3 days; refrigerator 3, 5, and 7 days; silica gel 7, 10, 20, and 30 days; and transport buffer 3, 5, and 7 days) and two leaf tissue maceration methods (liquid nitrogen and the TissueLyser®). Statistically significant differences in the obtained DNA concentration were found between the maceration and storage treatments. The TissueLyser® macerator produced higher DNA concentrations when compared to liquid nitrogen. For the storage treatments, five groups were formed based on DNA concentration when macerated with the TissueLyser® and two groups when macerated with liquid nitrogen. The DNA concentrations ranged from 285.00 ng/µL (7 days in transport buffer) to 702.00 ng/µL (30 days in silica gel) when the leaflets were macerated with liquid nitrogen, and from 572.73 ng/µL (30 days in silica gel) to 2,850.00 ng/µL (3 days in lyophiliser) using the TissueLyser® macerator. The DNA purity (A260/A280 nm) varied from 1.30 to 1.70 when the leaflets were macerated with liquid nitrogen and from 1.30 to 1.90 with the TissueLyser® macerator. Despite the variations in leaf tissue preservation and DNA concentration, all treatments were effective for DNA isolation and it was possible to amplify genomic regions of microsatellite markers by PCR. It was concluded that leaflets of E. precatoria stored in a lyophiliser and processed with an automatic macerator resulted in satisfactory DNA for molecular studies.
Highlights
The açaizeiro (Euterpe precatoria Mart.), popularly known as açaí-solteiro or açaí-doamazonas, is a single-stemmed palm tree belonging to the family Arecaceae
Five groups were formed based on DNA concentration when macerated with the TissueLyser® and two groups when macerated with liquid nitrogen
The DNA concentrations ranged from 285.00 ng/μL (7 days in transport buffer) to 702.00 ng/μL (30 days in silica gel) when the leaflets were macerated with liquid nitrogen, and from 572.73 ng/μL (30 days in silica gel) to 2,850.00 ng/μL (3 days in lyophiliser) using the TissueLyser® macerator
Summary
The açaizeiro (Euterpe precatoria Mart.), popularly known as açaí-solteiro or açaí-doamazonas, is a single-stemmed palm tree belonging to the family Arecaceae. It is found naturally in the northern region of Brazil, in the states of Acre, Rondônia, Amazonas, and Pará. The most utilised part is the mesocarp, from which a thick liquid known as "açaí juice" is extracted This liquid is very nutritious and has a high calorific value (YUYAMA et al, 2011). It is widely consumed in the Brazilian Amazon within all socioeconomic levels of the population. The exploitation of E. precatoria is still extractivism-based
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