Abstract
Mycorrhizal occurrence was tested in roots of 24 terrestrial and epiphytic orchid species growing at the Lankester Botanical Gardens (Cartago, Costa Rica). Standard staining and light microscopy were used to search for pelotons. Roots in contact with the substrate harbor extensive mycorrhyzal fungi. There may be a wide distribution and density of the fungal flora in the secondary forests of the site, this could explain the high natural regeneration of orchids on the garden.
Highlights
Mycorrhizal occurrence was tested in roots
epiphytic orchid species growing at the Lankester Botanical Gardens
light microscopy were used to search for pelotons
Summary
Jardín Botánico Lankester, Universidad de Costa Rica, Apdo. 1031-7050 Cartago, Costa Rica. Abstraet: Mycorrhizal occurrence was tested in roots of 24 terrestrial and epiphytic orchid species growing at the Lankester Botanical Gardens (Cartago, Costa Rica). De acuerdo con la localización de las hifas con respecto a las células, las micorrizas se cla sifican en ectomicorrizas y endomicorrizas. En las endomicorrizas el hongo crece dentro de las células corticales de la raíz y forma estructuras características (Currah 1991). Existen dos tipos de endomicorrizas, el grupo más común se distingue por presentar hifas aseptadas, vesículas y estructuras ramificadas que le confieren el nom bre de micorrizas vesícula-arbusculares. El segundo grupo está constituido por hongos con hifas septadas que invaden las cé lulas de la raíz sin romper la membrana plas mática y crecen dentro de la célula formando estructuras globosas conocidas como pelotones (Richardson et al 1993). Este tipo de micorriza es menos común y se encuentra solamente en las familias Ericaceae y Orchidaceae (Hadley 1982)
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