Presencia cualitativa y distribución de caveolina 1 (cav-1) en la celularidad y estadios del ciclo de la espermatogénesis

Abstract

The caveolins are plasma membrane domains involved in the sequestering of lipids and proteins. The caveolins have a pivotal role in lipid metabolism, growth regulation, signal transduction and apoptosis. Caveolins interact and regulate the heterodimers of protein G. Caveolin 1 and 2 are expressed abundantly in differentiated fibroblasts, smooth and skeletal muscle cells, endotheliocytes and adipocytes. ObjectiveThe aim is to demonstrate the presence of cav-1 in different cell populations of the seminiferous tubules in the testicle, according to the stage of the spermatogenesis cycle (mitosis, meiosis, and spermio-histogenesis) in the adult mouse. Material and methodsGonads were obtained from adult male mice (2.5 to 3 months). The testes were fixed immediately in buffered formalin (pH 7.2) for 48 hours. Impregnation with paraffin (melting point between 56°C to 58°C) was then performed using routine histological techniques. Sections of 5μ m were obtained and mounted on silanized slides for H&E staining and immunohistochemistry was performed with primary antibody against caveolin 1 (cav-1) and revealed with DAB. Results and discussionThe positive immunohistochemical reactions were analysed, and cav-1 positive cells were observed in the different cell populations of spermatogenesis (mitosis, meiosis and histogenesis), whose variations are restricted depending on the stage of the cycle of mouse spermatogenesis.