Abstract
1. 1. Two isozymes of adenylosuccinate synthetase, termed I and II have been separated by chromatography on DEAE-cellulose (DE-52), in Artemia embryos. 2. 2. The following data refer to form I and II: apparent molecular weights determined by sucrose gradient centrifugation and chromatography on Sephadex G-100: (I) 96,700, 90,000 and (II) 97,800, 83,000; K m values for their three substrates IMP, GTP and aspartate: (I) 26, 29, 600 and (II) 20, 25, 700 μM; fructose 1,6-biphosphate was a competitive inhibitor towards IMP with a K i value of 22 μM for both isozymes. 3. 3. The relationship between the Artemia enzyme and that from rat liver and other sources, is also discussed in this paper.
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