Presence of sibling during the learning phase of imprinting affects escape behavior from a new object in chicks

Abstract

Imprinting is a crucial learning behavior by the hatchlings of precocious birds. In nature, hatchlings in a group environment imprint on a hen, but the effect of siblings on the imprinting process remains largely unknown. To investigate this issue, we examined how the social context modulated visual imprinting in domestic chicks. One-day-old postnatal chicks in isolation (RS chicks) or with siblings (RD chicks), were first exposed to an imprinting stimulus, and subsequently the responses to the imprinting stimulus as well as a new stimulus were examined and compared. The experiment constituted three types of siblings: a 20-min pre-trained tutor, a 60-min pre-trained tutor, and a naïve chick. A multiple comparison test revealed that the preference score (PS) to the new stimulus of RD chicks trained with a 60-min pre-trained tutor was significantly lower than that of RS chicks. Multiple linear regression analysis revealed that the length of the tutor’s pre-training significantly correlated negatively with the PS to the new stimulus, but this variable did not correlate with the PS to the imprinting stimulus. These results revealed that the presence of highly imprinted siblings could enhance the escape response to the new stimulus. We discussed the possible involvement of the chick’s medial amygdala in the social aspect of imprinting.