Presence of pituitary adenylate cyclase activating polypeptide (PACAP) and its type I receptor in the rat kidney under normal and pathological circumstances

Abstract

Event Abstract Back to Event Presence of pituitary adenylate cyclase activating polypeptide (PACAP) and its type I receptor in the rat kidney under normal and pathological circumstances Andrea Lubics1*, Reka Brubel1, László Márk2, Dóra Reglodi1, Gabriella Horváth1, Andrea Tamás1, Péter Kiss1, Eszter László1, József Németh3 and Péter Szakály4 1 University of Pécs, Department of Anatomy, Hungary 2 University of Pécs, Department of Biochemistry and Medical Chemistry, Hungary 3 University of Debrecen, Department of Pharmacology and Pharmacotherapeutics, Hungary 4 University of Pécs, Department of Surgery, Hungary PACAP is a multifunctional neuropeptide, with two active forms, PACAP38 and PACAP27. It is now well-established that PACAP has several actions also in peripheral organs, including renoprotective effects. The peptide itself has not been identified in the rat kidney yet, and the exact localization of its specific receptor (PAC1R) is not yet known. The first aim of our study was to identify PACAP in the rat kidney using mass spectrometry and RIA. Our second aim was to identify the cell types where PAC1R is expressed in the rat kidney. We have previously shown that PACAP is protective in renal ischemia/reperfusion injury. Therefore, the third aim of the present study was to investigate the changes of endogenous PACAP following 60 min renal ischemia using RIA. Mass spectrometry revealed the presence of PACAP38 in the rat kidney. RIA measurements showed both PACAP38- and PACAP27-like immunoreactivities (IR) in kidney homogenates, with PACAP38 being dominant. Immunohistochemistry revealed PAC1 receptor-like IR in kidney sections, mainly expressed in cortical tubular epithelial cells. Changes in PACAP-like IR following renal vessel clamping were observed within 24 hours. In the cortex, an acute decrease was followed by an increase on the intact side and levels returned to original ones on the operated side. In the medulla, changes were only observed on the clamped side: a marked upregulation was detected in PACAP38-like IR within the first 24 hours. These results prove that PACAP is endogenously present in the kidney, and the tubular localization of PAC1 receptor provides the basis for the renal effects of the peptide under physiological and pathological conditions. The present study also showed that PACAP38- and PACAP27-like IR sensitively react to renal ischemia/reperfusion, the physiological importance of which awaits further investigation. Support: OTKA F67830, K72592, CNK78480, Richter Foundation, Bolyai Scholarship, PTE AOK Research Grant 2009, ETT 278-04/2009. Conference: IBRO International Workshop 2010, Pécs, Hungary, 21 Jan - 23 Jan, 2010. Presentation Type: Poster Presentation Topic: Homeostatic and neuroendocrine systems Citation: Lubics A, Brubel R, Márk L, Reglodi D, Horváth G, Tamás A, Kiss P, László E, Németh J and Szakály P (2010). Presence of pituitary adenylate cyclase activating polypeptide (PACAP) and its type I receptor in the rat kidney under normal and pathological circumstances. Front. Neurosci. Conference Abstract: IBRO International Workshop 2010. doi: 10.3389/conf.fnins.2010.10.00105 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 23 Apr 2010; Published Online: 23 Apr 2010. * Correspondence: Andrea Lubics, University of Pécs, Department of Anatomy, Pécs, Hungary, andrea.lubics@aok.pte.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Andrea Lubics Reka Brubel László Márk Dóra Reglodi Gabriella Horváth Andrea Tamás Péter Kiss Eszter László József Németh Péter Szakály Google Andrea Lubics Reka Brubel László Márk Dóra Reglodi Gabriella Horváth Andrea Tamás Péter Kiss Eszter László József Németh Péter Szakály Google Scholar Andrea Lubics Reka Brubel László Márk Dóra Reglodi Gabriella Horváth Andrea Tamás Péter Kiss Eszter László József Németh Péter Szakály PubMed Andrea Lubics Reka Brubel László Márk Dóra Reglodi Gabriella Horváth Andrea Tamás Péter Kiss Eszter László József Németh Péter Szakály Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.