Presence of multiple amylases encoded by independent genes in Cellulomonas sp. NCIM 2353

Abstract

Four genomic fragments encoding independent amylases from Cellulomonas sp. NCIM 2353 were cloned in Escherischia coli DH10B, using plasmid Bluescript. The enzyme encoded by these clones could be induced in the presence of starch, without IPTG. On zymogram amylase activity staining, two of these clones were seen to produce independent enzymes, corresponding to the Cellulomonas amylases. The restriction digestion profiles confirmed that these four DNA fragments are situated at different locations on the Cellulomonas genome. Some of the clones also harbored xylanase activity.