Abstract
With the widespread use of Roundup Ready soya (event 40-3-2) (RRS), the traceability of transgenic components, especially protein residues, in different soya-related foodstuffs has become an important issue. In this report, transgenic components in commercial soya (including RRS) protein concentrates were firstly detected by using polymerase chain reaction (PCR) and western blot. The results illustrated the different degradation patterns of the cp4-epsps gene and corresponding protein in RRS-derived protein concentrates. Furthermore, western blot was applied to investigate the single factor of food processing and the matrix on the disintegration of CP4-EPSPS protein in RRS powder and soya-derived foodstuffs, and trace the degradation patterns during the food production chain. Our results suggested that the exogenous full length of CP4-EPSPS protein in RRS powder was distinctively sensitive to various heat treatments, including heat, microwave and autoclave (especially), and only one degradation fragment (23.4 kD) of CP4-EPSPS protein was apparently observed when autoclaving was applied. By tracing the protein degradation during RRS-related products, including tofu, tou-kan, and bean curd sheets, however, four degradation fragments (42.9, 38.2, 32.2 and 23.4 kD) were displayed, suggesting that both boiling and bittern adding procedures might have extensive effects on CP4-EPSPS protein degradation. Our results thus confirmed that the distinctive residues of the CP4-EPSPS component could be traced in RRS-related foodstuffs.
Highlights
Roundup Ready soybean (RRS) is one of the major genetically modified crop species
Commercial soya protein concentrates were purchased from markets in different cities of China, among which only one sample from Anyang (Henan Province) was labeled as RRS protein concentrate
Our results further suggested that exogenous DNA degradation had been taken place during or after the processing of the protein concentrates (Figure 1a) as the band of full-length cp4-epsps gene (1584 bp) [13] only showed in the unprocessed RRS sample (RRS), while absent in all the five RRS protein concentrates and the unprocessed non-transgenic soya powder (Con)
Summary
Roundup Ready soybean (event 40-3-2) (RRS) is one of the major genetically modified crop species. Considering the significant importance of soya products in human nutrition, RRS is firmly established in the human diet and has gained great popularity in the global market, especially in China [2]. The main food products and food ingredients derived from soybean in China are soya-derived products, such as tofu, tou-kan and bean curd sheets, as well as soya protein concentrates or isolates used in the food industry. To ensure food safety control, all the foodstuffs derived from genetically modified technology must undergo a comprehensive evaluation before entering the market. Such assessment is of legal importance as part of the regulatory approval [3]
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