Abstract
A novel Streptomyces, strain MUSC 149T was isolated from mangrove soil. A polyphasic approach was used to study the taxonomy of MUSC 149T, which shows a range of phylogenetic and chemotaxonomic properties consistent with those of the members of the genus Streptomyces. The diamino acid of the cell wall peptidoglycan was LL-diaminopimelic acid. The predominant menaquinones were identified as MK9(H8) and MK9(H6). Phylogenetic analysis indicated that closely related strains include Streptomyces rhizophilus NBRC 108885T (99.2% sequence similarity), S. gramineus NBRC 107863T (98.7%) and S. graminisoli NBRC 108883T (98.5%). The DNA–DNA relatedness values between MUSC 149T and closely related type strains ranged from 12.4 ± 3.3% to 27.3 ± 1.9%. The DNA G + C content was determined to be 72.7 mol%. The extract of MUSC 149T exhibited strong antioxidant activity and chemical analysis reported identification of an antioxidant agent, Pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-. These data showed that metabolites of MUSC 149T shall be useful as preventive agent against free-radical associated diseases. Based on the polyphasic study of MUSC 149T, the strain merits assignment to a novel species, for which the name S. mangrovisoli sp. nov. is proposed. The type strain is MUSC 149T (=MCCC 1K00699T=DSM 100438T).
Highlights
Oxidative stress has been implicated in physiological aging which may contribute to the development of chronic diseases
Isolation and Maintenance of Isolate Strain MUSC 149T was isolated from a soil sample collected at site MUSC-TLS1 (3◦ 48 3.2 N 103◦ 20 11.0 E), located in the mangrove forest of Tanjung Lumpur in the state of Pahang, Peninsular Malaysia, in December 2012
The morphological observation of a 15-day-old culture grown on ISP 2 agar revealed
Summary
Oxidative stress has been implicated in physiological aging which may contribute to the development of chronic diseases. Many members of this genus have made vital contributions to mankind due to their capabilities to produce various natural products (Berdy, 2005) These Streptomyces-derived secondary metabolites have attracted much attention from the community as they possess diverse bioactivities such as antibacterial, antifungal, antitumor, and antioxidant (Kaneko et al, 1989; Kim et al, 2008; Olano et al, 2009a; Saurav and Kannabiran, 2012; Thenmozhi and Kannabiran, 2012; Wang et al, 2013; Kumar et al, 2014; Khieu et al, 2015). The outcomes derived from this research have provided a strong foundation for further in depth biological studies to be performed focusing on free-radical associated diseases
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