Abstract
Purpose:Bignonia capreolata is a perennial semi-evergreen vine from the Eastern US that was used as medicine by the Native Americans but has since fallen out of use. The aim of this analysiswas to 1) verify the presence of the indole alkaloid reserpine in B. capreolata; and 2) if verified, generate an alkaloid rich fraction of B. capreolata to more accurately identify and quantify reserpine. Methods: Initial confirmation of the presence of reserpine was performed using high performance liquid chromatography referenced with an analytical standard of reserpine. The alkaloid rich fraction was then tested via liquid chromatography and mass spectrometry. Results:Thepresence of reserpine in B. capreolata has been confirmed and found to be 49 g per gram of leaf. Conclusion: This is the only known plant outside of the Apocynacea plant family to contain this indole alkaloid. Contact: Trevor Clark, tclark@bastyr.edu
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