Abstract
Aim of study: To evaluate the effects of two different prescribed burning strategies on ectomycorrhizal (ECM) fungal species in Pinus cubensis-dominated natural forest.
 Area of study: Yateras Silvicultural Base Business Unit, Guantánamo, Cuba.
 Material and methods: In June 2015, six plots (20 × 50 m) were subjected to forward or back burning. Nine interval samplings (performed 1 week before and up to 120 days after prescribed burning) were undertaken to determine the total number of sporocarps and to evaluate the effect of fire on the soil.
 Main results: Eight ECM species were collected from the study plots. Suillus sp. and Amanita muscaria started fruiting 15 and 60 days after the fire, respectively. Boletus sp., Suillus brevipes, Suillus decipiens, Suillus sp., Amanita muscaria, Lactarius semisanguifluus, Scleroderma stellatum and Pisolithus arhizus were found before and after prescribed burning. Sporocarp numbers showed an increasing trend after fire and significantly recovered 75 days after forward or back burning and were significantly higher 120 days after forward burning compared to unburned plots. The ECM fungal community in the heading fire and the backfire plots did not differ significantly. However, non-metric multidimensional scaling confirmed that ECM composition differed over time. According to a Mantel test, the sampling time after prescribed burning accounted for 64% of the variation in ECM composition, followed by edaphic factors (26%) such as organic matter and Na.
 Research highlights: This preliminary study suggests that low-intensity prescribed burning does not have a negative effect on ECM fungal dynamics in humid tropical forests.
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