Preprotection of Tea Polysaccharides with Different Molecular Weights Can Reduce the Adhesion between Renal Epithelial Cells and Nano-Calcium Oxalate Crystals.

Yao-Wang Zhao,Chuang-Ye Li,Li Liu,Xin-Yuan Sun,Hui Zhang,Jian-Ming Ouyang

doi:10.1155/2020/1817635

Yao-Wang Zhao, Chuang-Ye Li + Show 4 more

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https://doi.org/10.1155/2020/1817635

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Abstract

Crystal adhesion is an important link in the formation of kidney stones. This study investigated and compared the adhesion differences between nano-calcium oxalate monohydrate (COM) and human renal proximal tubule epithelial (HK-2) cells before and after treatment with tea polysaccharides (TPSs) TPS0, TPS1, TPS2, and TPS3 with molecular weights of 10.88, 8.16, 4.82, and 2.31 kDa, respectively. TPS treatment effectively reduced the damage of COM to HK-2 cells, thereby resulting in increased cell activity, decreased release of lactate dehydrogenase, cell morphology recovery, decreased level of reactive oxygen species, increased mitochondrial membrane potential, increased lysosomal integrity, decreased expression of adhesion molecule osteopontin and eversion of phosphatidylserine, and decreased crystal adhesion. Among the TPSs, TPS2 with moderate molecular weight had the best protective effect on cells and the strongest effect on the inhibition of crystal adhesion. Thus, TPS2 may be a potential anticalculus drug.

Highlights

Tea is one of the most popular drinks in the world [1] and has many variations, such as green, black, oolong, and Pu’er tea
In our previous study [13], we investigated the antioxidant activities of four green tea polysaccharides (TPSs) with different molecular weights (10.88 (TPS0), 8.16 (TPS1), 4.82 (TPS2), and 2.31 kDa (TPS3)) and their repair of damaged human renal proximal tubule epithelial (HK-2) cells
We have found that polysaccharides extracted from green tea [13, 17] and Porphyra yezoensis [18] have the ability of repairing damaged renal epithelial cells

Summary

Introduction

Tea is one of the most popular drinks in the world [1] and has many variations, such as green, black, oolong, and Pu’er tea. Different concentrations (0.00078–5 μg/mL) of black tea extract (BTE) have toxic effects on human colon cancer cells (HT-29), human breast cancer cells (MCF-7), and human alveolar cancer cells (A549) and have no effect on normal cells (NIH-3T3) [4]. Fei et al [6] observed the that water extract of Pu’er tea, BT, and green tea increased the lifespan of worms, postponed Aβ-induced progressive paralysis of Alzheimer’s disease in transgenic worms, and improved the tolerance of worms to oxidative stress induced by heavy metal Cr6+

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