Abstract
Developing cancer immunotherapies relies on an accurate understanding of the diversity of cancer antigen–MHC complexes presented by immune cells, yet popular in vitro cell culture systems do not fully replicate the native tumor microenvironment. To characterize the cancer antigen fragments presented by immune cells in vivo, the authors engineered a mouse model with an affinity tag linked to the MHC heavy chain protein H2-Kb, enabling cell type-specific purification of cancer antigen–MHC complexes. In a mouse model of lung adenocarcinoma, this system revealed context-dependent changes in the peptide signatures obtained at different stages of tumorigenesis, including potential immunotherapeutic targets. Overall, this model offers a new way for the scientific community to evaluate in vivo immune cell interactions that is applicable across multiple cancer and disease settings.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callSimilar Papers
More From: Molecular biology of the cell
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
