Preparing nano silver particles (Ag NPs) by laser and studying the possibility of diagnosing cancer

Abstract

This research aims to prepare nanosilver (AgNPs) solution by using an Nd: YAG laser and implement it in diagnosing tumors in different vitro-tissues. Various properties of nano and coated particles were studied as well as the properties of used Nd: YAG laser. Then, the various tissues were studied by different microscopes before and after treatment. This research was carried out on two stages, the first stage included preparing the nanoparticles by exposing the pure silver target (99.98%) to laser Nd: YAG of (1000 mjoule and 6 Hz), where the light was centered on the silver target by exploitation the lens of focal length (ten cm). within the second stage, silver nanoparticals were used to diagnose the infected tissues by using a laser. At first, the nano silver solution was injected into the infected tissues then exposing them to continuous laser diode of ( 410nm) and power of (500 m watt) and take an image by transmission microscope and reflection microscopy of the tissues before and after treatment. The optical and structural properties of nano silver solution were studied by using FTIR, XRD,(UV-VIS spectrophotometer), optical, AFM and SEM microscopes. Images of transmission and reflection microscopes show a noticeable change in tissue morphology. Where most of the tissue spaces were filled with nano solution and an outstanding glowing appeared after injection which good evidence for the possibility of using this solution in diagnosing the infected parts of the tissue.