Preparative separation of phloridzin from apple leaves using macroporous resins followed by preparative high-performance liquid chromatography.

Abstract

Phloridzin is one of the major phenolic compounds in apple and has been widely used in medicine for a long time due to its significant biomedical activities. In this article, macroporous resin was used for purification of phloridzin from apple leaves. The HPD-300 resin was selected for the enrichement of phloridzin according to its high adsorption and desorption capacities. The adsorption kinetics and isotherms were constructed on the HPD-300 resin and fitted well to the pseudo-second-order kinetic model and Langmuir model, respectively. Dynamic adsorption and desorption tests were performed on the column packed with HPD-300 resin to optimize the operating parameters. After one round treatment with HPD-300 resin, the purity of phloridzin in the product increased from 11.4 to 50.1% with a recovery yield of 79.3%. Subsequently, preparative high-performance liquid chromatography was employed for the purification of phloridzin. The purity of phloridzin could reach above 98% after further recrystallization with a recovery yield of 75.8%.