Abstract
Centrifugal partition chromatography (CPC) allowed purification of anthocyaanins from Champagne vintage by-products ( Vitis vinifera) and from blackcurrant ( Ribes ni9grum L.). In the first case a 5-1 pilot-CPC used on a preparative multi-gram scale. The biphasic solvent system used was ethyl acetate-1-butanol-water, acidified by TFA (pH 1–3) for both gradient and isocratic normal-phase elutions. Separation selectives which differ for cyanidin and delphinidin glycosides in CPC and cellulose-TLC using the solvent system 1-butanol-acetic acid-water (4:1:5) are discussed.
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