Abstract
The contamination of foods and animal feeds with trichothecene mycotoxins is a growing concern for human and animal health. As such, large quantities of pure trichothecene mycotoxins are necessary for food safety monitoring and toxicological research. A new and effective method for the purification of trichothecene mycotoxins from a marine fungus, Fusarium sp. LS68, is described herein. Preparative high-speed countercurrent chromatography (HSCCC) was utilized for the scalable isolation and purification of four trichothecene mycotoxins for the first time in stepwise elution mode, with a biphasic solvent system composed of hexanes–EtOAc–CH3OH–H2O (6:4:5:5, v/v/v/v) and (8.5:1.5:5:5,v/v/v/v). This preparative HSCCC separation was performed on 200 mg of crude sample to yield four trichothecene mycotoxins, roridin E (1), roridin E acetate (2), verrucarin L acetate (3), and verrucarin J (4) in a single run, with each of >98% purity. These compounds were identified by MS, 1H NMR, 13C NMR, and polarimetry. The results demonstrate an efficient HSCCC method for the separation of trichothecene mycotoxins, which can be utilized to produce pure commercial and research standards.
Highlights
According to the Food and Agriculture Organization of the United Nations, about 25% of the food crops in the world are contaminated with mycotoxins, and these have adverse effects on humans, animals, and crops that result in serious illnesses and economic losses [1]
Among the major mycotoxins produced by Fusarium species, trichothecenes are pathogenic to important agricultural crops and foods [2], and these lead to serious economic losses by reducing yields and overall quality of North American agricultural products [3,4]
Trichothecenes are a group of tetracyclic sesquiterpene mycotoxins that are produced by various fungi from the order Hypocreales, including those of the genera Fusarium, Myrothecium, Mar
Summary
According to the Food and Agriculture Organization of the United Nations, about 25% of the food crops in the world are contaminated with mycotoxins, and these have adverse effects on humans, animals, and crops that result in serious illnesses and economic losses [1]. These molecules are potent phytotoxins, and act as the virulence factors of pathogenic fungi on sensitive. Are isolated from fungal extracts by conventional methods, macroporous resin separation trichothecene mycotoxins are isolated from fungal extractssuch by as conventional methods, such as followed by silica column followed chromatography. Methods can be and time-consuming, and result in substantial sample loss due to irreversible adsorption considered costly, laborious, and time-consuming, and result in substantial sample loss to thetosilica [16,17] It is thereforetoimportant develop rapid and efficient methods for the separation due irreversible adsorption the silica to [16,17]. HSCCC has been for used the preparative separation adsorption bysolid solid support media haswidely been used widely for the preparative and purification of natural products, this is aand scalable technology.
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