Abstract
The use of macroporous resins for the separation and purification of total flavonoids to obtain high-purity total flavonoids from Scorzonera austriaca was studied. The optimal conditions for separation and purification of total flavonoids in S. austriaca with macroporous resins were as follows: D4020 resin columns were loaded with crude flavonoid extract solution, and after reaching adsorptive saturation, the columns were eluted successively with 5 bed volumes (BV) of water, 5 BV of 5% (v/v) aqueous ethanol and 5 BV of 30% (v/v) aqueous ethanol at an elute flow rate of 2 BV·h−1. Total flavonoids were obtained from the 30% aqueous ethanol eluate by vacuum distillation recovery. The content of flavonoid compounds in the total flavonoids was 93.5%, which represents an improvement by about 150%. In addition, five flavonoid compounds in the product were identified as 2″-O-β-d-xylopyranosyl isoorientin, 6-C-α-l-arabipyranosyl orientin, orientin, isoorientin and vitexin by LC-ESI-MS analysis and internal standard methods. The results in this study could represent a method for the large-scale production of total flavonoids from S. austriaca.
Highlights
Scorzonera austriaca Wild, a perennial herb of the Compositae, is widely distributed in the northeast and northwest regions in China, being especially abundant in Jilin Province
Where Qa is the adsorption capacity at adsorption equilibrium; Qd is the desorption capacity after adsorption equilibrium; Rd is the desorption ratio (%); C0 and Ce are the initial and equilibrium concentrations of total flavonoids in the solution, respectively; Cd is the concentration of total flavonoids in the desorption solution; Vi is the volume of the initial sample solution; Vd is the volume of the desorption solution; W is the weight of resin (g)
A 1.40 mgmL1 crude flavonoid extract solutionfrom Scorzonera austriaca was used as the test sample
Summary
Scorzonera austriaca Wild, a perennial herb of the Compositae, is widely distributed in the northeast and northwest regions in China, being especially abundant in Jilin Province. It has been widely used as a traditional herbal medicine for curing fevers, carbuncles and mastitis [1]. To date many methods for flavonoid purification have been developed. These include high speed counter-current chromatography [5], solvent extraction, agarose gel media [6], supercritical carbon dioxide extraction [7], aqueous two-phase flotation and molecule imprinted solid phase extraction [8,9]
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