Abstract
We successfully used dual-mode centrifugal partition chromatography (CPC) to separate and purify major components from an ethyl acetate extract of Parthenocissus tricuspidata stems. Dual-mode CPC with a solvent system composed of ethyl acetate–acetonitrile–water (3:3:4, v/v) was performed in reverse-phase mode followed by normal-phase mode, through the use of a switching valve from descending to ascending mode. In reverse-phase mode (descending mode), more polar components such as aromadendrin-3-O-β-d-glucopyranoside (1), trans-piceid (2), and catechin (3) were isolated. Switching to normal-phase mode (ascending mode) yielded the less polar compounds, resveratrol (4) and engeletin (5). As a result, five major compounds, aromadendrin-3-O-β-d-glucopyranoside (46.6mg), trans-piceid (13.5mg), catechin (42.0mg), resveratrol (13.9mg), and engeletin (17.6mg), were isolated from 500mg of P. tricuspidata ethyl acetate extract. The purity of each isolated compound was determined to be 95% (1), 85% (2), 90% (3), 90% (4) and 95% (5) based on the HPLC peak areas.
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