Abstract
A fast and simple solvent system screening method was established based on average polarity, which was much easier to find a suitable solvent system for high-speed counter-current chromatography (HSCCC) separation. Six alkaloids were separated and purified simultaneously with n-hexane–ethyl acetate–methanol–water (2:5:2:5) on HSCCC in one step. As a result, 4.7mg palmatine, 7.1mg berberine, 0.8mg worenine, 1.5mg epiberberine, 1.9mg coptisine and 0.6mg jatrorrhizine from 20mg Rhizoma coptidis extract were obtained with the purities of 98.5%, 94.1%, 90.4%, 95.5%, 88.4% and 91.1% respectively as determined by high-performance liquid chromatography (HPLC), and they were characterized by MS and 1H NMR.
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