Abstract
High speed countercurrent chromatography (HSCCC) coupled with an evaporative light scatter detector (ELSD) was successfully applied to preparative isolation and purification of β-caryophyllene from essential oil of Vetix negundo L. var. heterophylla (Franch.) Rehd. Using either n-hexane-chloroform-acetonitrile (6:2:5) or n-hexane-dichloromethane-acetonitrile (10:3:7) as the two-phase solvent system, β-caryophyllene was separated in one run from the crude essential oil by HSCCC. However, the application of n-hexane-dichloromethane-acetonitrile (10:3:7) resulted in a higher purity and recovery of β-caryophyllene. About 85 mg of β-caryophyllene representing 97.3% by GC, was yielded from 600 mg crude essential oil when eluted with the lower phase at a flow rate of 1.5 mL.min−1.
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