Abstract
The aim of this study was the purification process of polyphenols from Aronia melanocarpa (chokeberry), and the purification parameters were optimised by adsorption and desorption tests. By comparing adsorption and desorption ability of polyphenols from chokeberry on six kinds of macroporous resin, XAD-7 resin was selected. Experiments prove that the best purification parameters of static adsorption and desorption were sample pH = 4.0 with 4 h of adsorption; and desorption solvent is 95% ethanol (pH = 7.0) with 2 h of desorption. The best dynamic parameters were 9.3 bed volume (BV) of sample loading amount at a feeding flow rate of 2 BV/h, and washing the column with 5.8 BV of water, followed by subsequent elution with an eluent volume of 5.0 mL at an elution flow rate of 2 BV/h. Next the antioxidant and antiproliferative activity of polyphenols from chokeberry, blueberries, haskap berries was studied on HepG2 human liver cancer cells. The results show that polyphenol from chokeberry has a strong antioxidant effect. Taking into account the content of polyphenols in fruit, polyphenols from chokeberry represent a very valuable natural antioxidant source with antiproliferative products.
Highlights
Aronia melanocarpa (Rosaceae), called chokeberry, is the small berry native to North America that has a high economic value
Six macroporous resins with different adsorption capacity and desorption capacity were investigated for the separation and purification of chokeberry polyphenols
XAD-7 macroporous resin was selected as the best purification material
Summary
Aronia melanocarpa (Rosaceae), called chokeberry, is the small berry native to North America that has a high economic value. The flesh is deep red with a bitter and astringent taste. Chokeberry fruit is a dark blue and round berry. Chokeberries is used for processing into fruit jams, fruit wines, fruit juices, dried fruit, canned-foods and other products. Plant polyphenols are known for their unique antioxidant and antiproliferative activities. There is a great difference between the components and the content of polyphenols in different kinds of plants. Due to its rich content of polyphenols, berries can be a good source of natural antioxidants [1,2,3]
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