Abstract
In order to separate epigallocatechin-3-gallate (EGCG) from tea polyphenols, the adsorption ability of resins chosen toward catechins was investigated. According to static adsorption results of resins selected, polyamide displayed better selective adsorption ability between esterified and non-esterified catechins, and LSA-10 distinguished EGCG from ECG very well among esterified catechins. Therefore, columns packed with polyamide and resin LSA-10 were adopted in the first and second purification of EGCG, respectively. After the first-run purification, the product can be obtained with a 70.27 % EGCG purity and 80.21 % recovery. After the second-run separation, product contained an EGCG purity of 95.14 % and a total recovery of 76.78 % can be achieved. Moreover, a higher per volume adsorption material load amount 51.02 mg mL−1 tea polyphenols and yield 24.11 mg mL−1 EGCG were obtained in the study. Furthermore, only ethanol solution was used as elution reagent in the process. In the study, a method that is environmentally friendly, with lower cost and better repeatability has been developed. This result will be of help in development of tea resources and applications on a large scale.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
