Abstract
Purification of 1:1 adducts (enaminals) formed from the reaction of methyl esters of amino acids with either malondialdehyde or methylmalondialdehyde was accomplished by preparative liquid chromatography on the stationary phase Amberlite XAD-4. The enaminal was easily and rapidly separated from the by-products and starting materials by a EtOH-H 2O mobile phase and then easily and rapidly recovered from the EtOH-H 2O at good yield and high purity. Depending on column diameter, up to 50 mg of enaminal could be purified in one chromatographic step. Adducts derived from l-Arg, l-His, l-Tyr, and l-Cys were purified.
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