Abstract
Two prenylated biflavonoids, podoverines B–C, were isolated from the dried roots and rhizomes of Sinopodophyllum emodi using a Sephadex LH-20 column (SLHC) and high-speed counter-current chromatography (HSCCC). The 95% ethanol extract was partitioned with ethyl acetate in water. Target compounds from the ethyl acetate fraction were further enriched and purified by the combined application of SLHC and HSCCC. n-Hexane–ethyl acetate–methanol–water (3.5:5:3.5:5, v/v) was chosen as the two phase solvent system. The flow rate of mobile phase was optimized at 2.0 mL·min−1. Finally, under optimized conditions, 13.8 mg of podoverine B and 16.2 mg of podoverine C were obtained from 200 mg of the enriched sample. The purities of podoverines B and C were 98.62% and 99.05%, respectively, as determined by HPLC. For the first time, podoverins B and C were found in the genus Sinopodophyllum. Their structures were determined by spectroscopic methods (HR-ESI-MS, 1H-NMR, 13C-NMR, HSQC, HMBC). Their absolute configurations were elucidated by comparison of their experimental and calculated ECD spectra. The cytotoxic activities were evaluated against MCF-7 and HepG2 cell lines. The separation procedures proved to be practical and economical, especially for trace prenylated biflavonoids from traditional Chinese medicine.
Highlights
Sinopodophyllum emodi (Wall.) Ying, which belongs to the family of Berberidaceae, is a herbaceous perennial plant widely distributed in the Southwest of China [1]
The roots and rhizomes of S. emodi is rich in aryltetralin lactone lignans and normal flavonoids [22]
Based on the HPLC analysis, it was almost impossible for podoverines B and C to be detected in the ethyl acetate fraction
Summary
Sinopodophyllum emodi (Wall.) Ying, which belongs to the family of Berberidaceae, is a herbaceous perennial plant widely distributed in the Southwest of China [1]. [4], and and pyogenic infection of skin tissue [5]. Previous chemical investigations on S. emodi revealed the presence of bioactive infection of skin tissue [5]. Previous chemical investigations on S. emodi revealed the presence of aryltetralinaryltetralin [1,3,4,5,6,7,8] and[1,3,4,5,6,7,8]. Tetrahydrofuranoid lignans [9], flavonoids steroids [13], and phenolics bioactive and tetrahydrofuranoid lignans [9],[2,10,11,12], flavonoids [2,10,11,12], steroids [13], [14]. Tetrahydrofuranoid lignans [9], flavonoids steroids [13], and phenolics bioactive and tetrahydrofuranoid lignans [9],[2,10,11,12], flavonoids [2,10,11,12], steroids [13], [14]. phenolics [14].
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have