Abstract
The aim of this paper was to seek an efficient method to preparative separation of alkaloid compounds from Aconitum coreanum (Guanbaifu), a well-known traditional Chinese medicinal plant for heart disease. Seven alkaloid compounds were successfully purified by pH-zone-refining counter-current chromatography with two-phase solvent system of petroleum ether–ethyl acetate–methanol–water (5:5:1:9, v/v/v/v), 10 mM triethylamine in upper phase and 10 mM hydrochloric acid in lower phase. From 3.5 g of crude extract, 356 mg of Guanfu base I, 578 mg of Guanfu base A, 74 mg of atisine, 94 mg of Guanfu base F, 423 mg of Guanfu base G, 67 mg of Guanfu base R and 154 mg of Guanfu base P were obtained with the purity of 96.40%, 97.2%, 97.5%, 98.1%, 98.9%, 98.3% and 98.4%. Their chemical structures were identified by TOF-MS and 1H-NMR. This study indicated that pH-zone-refining counter-current chromatography was an efficient method for separating the kind of alkaloids with low absorbance values.
Highlights
Aconitum coreanum (Lèvl) Rapaics (Guanbaifu in Chinese) grows in Primorskii Krai, south China, and Korea [1]
The early research showed that Guanfu base A (GFA) could effectively terminate the fibrillation induced by bilateral vagus nerve stimulation with the total effective rate of 87.5% [5], it has strong antiarrhythmic effect and can effectively bind atrial fibrillation [5,6]
PH-zone-refining counter-current chromatography which was a liquid-liquid chromatography introduced by Ito [7,8] is a very good solution to this problem, as the main alkaloids can be separated by little peaks which are formed by minor impurities
Summary
Aconitum coreanum (Lèvl) Rapaics (Guanbaifu in Chinese) grows in Primorskii Krai, south China, and Korea [1]. The early research showed that GFA could effectively terminate the fibrillation induced by bilateral vagus nerve stimulation with the total effective rate of 87.5% [5], it has strong antiarrhythmic effect and can effectively bind atrial fibrillation [5,6]. It has been developed into a neo-type antiarrhythmic drug. This paper aims to report the advantages of these technologies, namely pH-zone-refining
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