Abstract
A preparative high speed counter-current chromatography (HSCCC) method for isolation and purification of flavonoid compounds from Scutellariae Radix, the roots of Chinese medicinal plant Scutellaria baicalensis Georgi, was successfully established by using ionic liquids as the modifier of the two-phase solvent system. Baicalin and wogonoside were purified from the crude extract of Scutellariae Radix by using ethyl acetate-water-[C4mim][PF6] (5:5:0.2, v/v) as two-phase solvent system. From 120 mg of the crude sample, 50.1 mg of baicalin and 45.6 mg of wogonoside were obtained in a one step separation. The purities of baicalin and wogonoside were 99.3% and 99.1%, respectively, as determined by HPLC area normalization method. The chemical structures of the isolated compounds were identified by1H-NMR and 13C-NMR.
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