Abstract
High-speed counter-current chromatography was applied in the isolation and purification of four isoflavonoids from the Chinese medicinal plant Belamcanda chinensis(L.) DC. Preparative high-speed counter-current chromatography method with two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water at volume ratio of 5:5:3:7 was successfully established, which yielded 6.2 mg of tectoridin, 8.5 mg of iridin, 22.6 mg of irigenin and 13.4 mg of irisflorentin from 150 mg of the crude extract in one-step separation. The purities were 98.1%, 98.9%, 99.0% and 99.2%, respectively, as determined by high performance liquid chromatography and their chemical structures were identified by 1H-NMR and C-NMR.
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