Preparative isolation and purification of five compounds from the Chinese medicinal herb Polygonum cuspidatum Sieb. et Zucc by high-speed counter-current chromatography

Abstract

High-speed counter-current chromatography (HSCCC) was applied to the separation and purification of five compounds from the Chinese medicinal herb Polygonum cuspidatum Sieb. et Zucc. The crude extracts from P. cuspidatum Sieb. et Zucc were treated with light petroleum–ethyl acetate–methanol–water (2:5:4:6, v/v). Sample 1 was obtained from the lower phase and sample 2 from the upper phase. The sample 1 was separated with light petroleum–ethyl acetate–water (1:5:5, v/v) and yielded 19.3 mg of piceid, 17.6 mg of anthraglycoside B from 200 mg of sample 1. The sample 2 was separated with light petroleum–ethyl acetate–methanol–water (3:5:4:6, v/v) and light petroleum–ethyl acetate–methanol–water (3:5:7:3, v/v) in a gradient elution and yielded 18.5 mg of resveratrol, 35.3 mg of emodin and 8.2 mg of physcion from 220 mg of sample 2. The purity of each compound is over 95% as determined by HPLC. The chemical structures of these components were identified by 1H NMR and 13C NMR.