Abstract
Capability of a new countercurrent chromatographic scheme is demonstrated on preparative-scale separation with two typical two-phase solvent systems. The scheme uses a helical column revolving around the horizontal axis of the centrifuge and rotating about its own axis at the same angular velocity. This planetary motion while eliminating the need for the rotating seals, enables utilization of the gravitational and/or centrifugal force fields to perform a high-efficiency countercurrent chromatography with a variety of two-phase solvent systems.
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