Preparation of Zr4+ affinity column by atom transfer radical polymerization for phosphoprotein isolation

Abstract

AbstractThis paper describes the preparation of the metallic affinity column by atom transfer radical polymerization of glycidyl methacrylate (GMA) on Wang resin surface. The metallic ions (Zr4+, Fe3+, and Pd2+) were introduced after phosphonation of the epoxy group of the grafted poly(GMA) on Wang resin surface. The successful results on metallic ion‐immobilized polymeric microsphere stationary phase were confirmed via Fourier transform infrared, scanning electron microscopy, X‐ray photoelectron spectra (XPS), and inductively coupled plasma‐atomic emission spectrometer. The resolution degree of the phosphonated casein and dephosphonated casein for Zr4+, Fe3+, and Pd2+ affinity column was examined. The result shows that the resolution time (min) of the phosphonated casein was higher than that of the dephosphated casein on the Zr4+ affinity column by liquid chromatography. However, the phosphonated casein and dephosphonated casein were not separated on the Fe3+ affinity column and Pd2+ affinity column by liquid chromatography. © 2009 Wiley Periodicals, Inc. J Appl Polym Sci, 2009