Preparation of Withaferin A nanoparticles extracted from Withania somnifera by the expansion of supercritical fluid solution

Abstract

Withania somnifera (L.) Dunal. is a plant with several important medicinal properties that have long been used in traditional therapy to treat some diseases. Micronisation reduces the particle size, which increases the bioavailability. In this study, due to the great potential of Withaferin A in the treatment of diseases, the nanoparticle formation of Withaferin A extracted from Withania somnifera, was considered. In the first step, the experimental parameters of supercritical fluid extraction of W. somnifera were optimised by central composite design (CCD). Then, the herbal extract was micronised using a new, repeatable, and robust method in terms of the expansion of carbon dioxide supercritical solvent. Also, the parameters of the experiment were optimised with the Draper-Lin small composite designs. Moreover, we identified Withaferin A nanoparticle in the extracted samples by utilising liquid chromatography-mass spectrometry (LC-MS) and the obtained precipitates were characterised using field emission scanning electron microscopy (FESEM). The optimal conditions of the experiment were as follows: pressure drop 254 atm, at the temperature of 53°C, equilibrium time 23 min, and collection time 57 min. Based on the observed results, the optimum points for the size and number of Withaferin A nanoparticles were predicted as 5 and 5842 nm, respectively. The nanoparticle production was accomplished through the expansion of supercritical solution, while the speed of expansion was much lower compared to the ordinary rapid expansion of supercritical solution (RESS) methods. Also, the nanonisation conditions, especially the pressure drop, significantly affected the formation of nanoparticles.