Abstract
(1→3)-β-D-glucan from the inner cell wall of Saccharomyces cerevisiae is considered a member of a class of drugs known as biological response modifiers (BRM). However the glucan was an insoluble polysaccharide, which could be the major barrier to the utilization of glucan. In this case, the insoluble glucan was convent into a soluble form by four kind of solubilizing processes. The yield, solubility, chemistry structure and immunoprophylaxis efficacy of the soluble products were compared. Our date suggest that urea has a significant effect on yield, and DMSO has a significant effect on solubility. FT-IR spectra, 13C NMR spectra and helix-coil transition analysis demonstrate that the chemistry structure of native and solubilizing glucans have no significant difference. They still have the triple helical structure. The solubility and immunoprophylaxis efficacy assay indicate that the introduction of phosphate group not only enhanced the solubility of glucan, but also improved the survival rate of mice challenged with E. coli.
Highlights
Glucan is a β-linked polyglucose immune stimulant that can be isolated from the cell wall of Saccharomyces cerevisiae [1,2]
dimethyl sulfoxide (DMSO) and urea was used as denaturant could facilitate the breakdown of the intermolecular hydrogen bonds between the glucan chains
The solubility of glucan C and D was significantly higher than glucan A and B, which could be attribute to the introduction of phosphate group
Summary
Glucan is a β-linked polyglucose immune stimulant that can be isolated from the cell wall of Saccharomyces cerevisiae [1,2]. It can be used as fat replacer, gelling agent, thickening agent [3,4,5,6]. Numerous studies have focused on converting these glucans into a water-soluble form through chemical modification such as amination [13], sulfation [14] and phosphorylation [15]. We have attempted to convert insoluble yeast glucans into a soluble form through phosphorylation process. Four different soluble products were compared by solubility, FT-IR spectra, 13C NMR spectra, and helix-coil transition analysis and immunoprophylaxis efficacy assay
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