Preparation of wall-coated open tubular glass (pyrex) capillary columns with polar stationary phases, using superox™-4 as a surface pretreating and deactivating agent

Abstract

Wall-coated open tubular (WCOT) glass (Pyrex) capillary columns were prepared with three different polar stationary phases (Carbowax 20M, Superox™-4, and SP-1000) using Superox-4 as a surface pretreating and deactivating agent. Methods for the laboratory preparation and practical application of these columns are given. Columns prepared by this technique were efficient, thermally stable, and low in surface activity. The Superox-4 pretreatment and deactivation technique produced high quality polar WCOT columns, and no surface roughening procedure was required. The technique offered a substantial decrease in column preparation time. In addition, columns prepared by this technique were comparable to those from commercial suppliers or those produced by conventional preparation techniques.