Abstract
Simple procedures for the preparation of UDP- d-[U- 14C]galacturonic acid and UDP- d-[6- 3H]galactose with high specific activities are described. The conversion involves the enzymatic oxidation of the appropriate UDP- d-galactose derivative to UDP- d-galactose-6-aldehyde followed by either further oxidation with iodine or reduction with sodium borotritide. The labeled products were isolated from reaction mixtures by ion exchange and paper chromatography.
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