Preparation of Tritium-Labeled Compounds II: Chlorphenesin Carbamate by Exposure to Tritium Gas and Determination of Intramolecular Distribution of Tritium

Abstract

Incorporation of stably bound tritium in purified chlorphenesin carbamate was 141 μ c. per curie-day exposure. The intramolecular distribution of radioactivity was determined by a combination of biological and chemical degradation methods. Aromatic substitution predominated, accounting for 95 per cent of the tritium; two-thirds of this was located ortbo to the chlorine substituent. The remaining 5 per cent of the radioactivity resided at the number one position of the alkoxy side chain.