Preparation of Transfer Ribonucleic Acid from Tobacco Plant and Examination of its Accepting Ability of Acetyl Serine

Abstract

Amino group of N-terminal serine of tobacco mosaic virus coat protein is known to be blocked by acetyl group, but the mechanism of acetylation has not been clarified. In this report, transfer RNA (tRNA) was prepared from tobacco plant (Nicotiana tabacum xanthi nc) and using this tRNA as an acceptor, whether acetyl serine can be transferred was examined by a competition method. Conclusion that acetyl serine is not transferred by a serine activation system was obtained.