Preparation of Symmetric Network PVDF Membranes for Protein Adsorption via Vapor-Induced Phase Separation

Abstract

Symmetric network poly(vinylidene fluoride) (PVDF) membranes without a dense skin layer were prepared by vapor-induced phase separation from a PVDF/N,N-dimethylacetamide (DMAc)/water system. The effects of evaporation atmosphere, temperature, and humidity during the preparation of the membranes on their morphologies were investigated by scanning electron microscope (SEM). With low temperature and high humidity, the polymer crystallization mechanism dominated the membrane formation process, and the casting solution formed membranes with symmetric morphologies in the vapor phase containing 0.79% DMAc. The effect of additives on the membrane structure and performance was also investigated. The results of adsorption experiments showed that the binding capacity of bovine serum albumin (BSA) increased with the appearance of a circular network morphology and the decrease of mean pore size of the membrane. With the addition of LiCl to the casting solution, the obtained membrane can adsorb BSA up to 150 μg/cm2. Proteins on sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis gels were successfully electro-blotted onto these PVDF membranes. Compared with commercial membranes, the PVDF membranes prepared in this work were more suitable for protein blotting.