Abstract
The NADH-dependent nitrate reductase from Fusarium oxysporum cell extract was directly immobilized as cross linked enzyme aggregates (CLEAs) and investigated for the synthesis of silver nanoparticles by a reduction of silver nitrate. The effects of precipitant type and cross-linking on activity recovery of enzyme in CLEAs were studied. After aggregation of enzyme with ammonium sulfate followed by cross-linking formed aggregates for 4h with 8mM glutaraldehyde, 93% activity recovery was achieved in CLEAs with enhanced thermal stability at 50°C and 40°C. Scanning electron microscopy analysis showed that immobilized NADH-dependent nitrate reductase was of spherical structure. CLEAs showed 90% catalytic yield even after 4cycles of repeated use in silver nanoparticle synthesis at pH7.2 and temperature 35°C.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
