Abstract
3-(1-Carboxypropyl) ether derivatives of 15α-hydroxyestradiol 15- N-acetylglucosaminide (15α-OHE 2 15NAG) and 15α-hydroxyestriol (E 4) 15NAG were synthesized and conjugated with bovine serum albumin. Antisera elicited in rabbits possessed high affinity and specificity for the 15α-hydroxyestrogen (15α-OHEs) 15NAG, exhibiting no significant cross-reactivity with 15α-OHEs and their positional isomers such as 16NAG and 17NAG. Enzyme immunoassay methods developed by using the purified antisera and horseradish peroxidase-labeled antigens were applied to the measurement of 15α-OHEs 15NAG and E 4 15NAG in normal pregnancy urine. We demonstrated for the first time that the conjugation of N-acetylglucosamine to E 4 occurs at the C-15α position.
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