Preparation of ( S)-mandelic acid from racemate using growing cells of Pseudomonas putida ECU1009 with ( R)-mandelate degradation activity

Abstract

( S)-Mandelic acid [( S)-MA] was prepared in a one-pot fermentation-transformation process by a ( R)-MA-degrading bacterium, Pseudomonas putida ECU1009. Using a sequential simplex optimization method, the best results were obtained with growing cells cultivated on a medium composed of 1.0% ( RS)-MA, 0.1% NH 4NO 3, and 0.1% corn steep liquor. The optimal cultivation conditions were 30 °C and pH 6.5. By fed-batch culture under the optimal conditions, ( S)-MA was successfully prepared in 1-L Erlenmeyer flask with 41% isolation yield and >99.9% ee, from totally 3% ( RS)-MA after 94 h of biotransformation.